Molecular characterization and genetic diversity of Jatropha curcas L. in Costa Rica

Vásquez-Mayorga, Marcela
Fuchs, Eric
Hernández, Eduardo
Herrera, Franklin
Hernández, Jesús
Moreira-González, Ileana
Arnáez-Serrano, Elizabeth
Barboza, Natalia

2017

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We estimated the genetic diversity of 50 Jatropha curcas samples from the Costa Rican germplasm bank using 18 EST-SSR, one G-SSR and nrDNA-ITS markers. We also evaluated the phylogenetic relationships among samples using nuclear ribosomal ITS markers. Non-toxicity was evaluated using G-SSRs and SCARs markers. A Neighbor- Joining (NJ) tree and a Maximum Likelihood (ML) tree were constructed using SSR markers and ITS sequences, respectively. Heterozygosity was moderate (He D 0.346), but considerable compared to worldwide values for J. curcas. The PIC (PIC D 0.274) and inbreeding coefficient (f D

Genetic diversity; Jatropha curcas; SCARs markers; EST-SSR; nrDNA-ITS region; Research Subject Categories::NATURAL SCIENCES::Biology::Cell and molecular biology::Molecular biology;