“Estudio de expresión de los genes cIAP y cMET, en tejido hepático con y sin neoplasia de Rattus Norvegicus”
2015-04-14T16:30:48Z
Instituto Tecnológico de Costa Rica. Escuela de Biología.
Hepatocellular carcinoma is the most common malignancy in the liver and
its incidence has doubled in the last 40 years, placing it as the fifth most
common tumor in the world. Despite the use of different methods of
diagnosis and treatment of this disease, none has proven completely
effective and are applicable only if the disease is diagnosed in its early
stages. Knowledge of the expression levels of genes involved in the
development of this cancer could bring new alternatives for prevention,
diagnosis and treatment of this disease, which is why the aim of this study
was to determine expression level of cMET and cIAP genes, in Rattus
norvegicus liver tissue with and without neoplasm. Using the technique of
reaction Polymerase Chain in real time (qPCR), we found that the
expression of both genes cIAP and cMET decreased in neoplastic
samples compared to samples not affected. We also analyzed the
expression of these genes in samples with neoplastic formations but
treated with an anti-tumor agent. The latter showed an increase in gene
expression cMET unlike the cIAP gene, which decreased its expression.
Because the number of samples used was small, no statistical analysis
was possible. However, these results do not coincide with those expected
theoretically.
Proyecto de Graduación (Bachillerato en Ingeniería en Biotecnología). Instituto Tecnológico de Costa Rica. Escuela de Biología, 2010.
Instituto Tecnológico de Costa Rica
Lidia Gómez
Cartago - 300m Este del Estadio Fello Meza. Apartado 159-7050.
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